Artikelnummer: 300154

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Human Neuroblastoma cell line SH-SY5Y

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Organism: Homo sapiens (human)
Age: 4 years old
Gender: Female
Tissue: Brain (from metastatic site: bone marrow)
Morphology: The cells grow as clusters of neuroblastic cells with multiple, short, fine cell processes (neurites). Cells will aggregate, form clumps and float; a confluent monolayer is not formed.
Cell type: Neuroblast (neuroblastoma)
Growth Properties: Loosely adherent; form clumps at high cell density
Description: SH-SY5Y is one of three serially isolated neuroblast clones (SH-SY, SH-SY5, SH-SY5Y) of the human neuroblastoma cell line SK-N-SH which was established in 1970 from a metastatic bone tumor. The cells exhibit moderate levels of dopamine beta hydroxylase activity. They can convert glutamate to the neurotransmitter GABA. SH-SY5Y cells have a reported saturation density greater than 1 x 106 cells/cm2. The loss of neuronal characteristics has been described with increasing passage numbers (approx. passage 20). Neuronal markers or uptake of noradrenalin should be determined routinely. It is recommended not to use at passage numbers higher than 20 unless neuronal characteristics are controlled.
Virus: The presence of SMRV in SH-SY5Y was detected at CLS by qPCR.
Biosafety level: Although there is no incidince of infections of hosts other than Squirrel Monkey, it is recommended to handle SH-SY5Y cells as a potentially biohazardous material under Biosafety Level 2 containment.