Artikelnummer: 300302

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Human Colon adenocarcinoma cell line
Frozen cryovial
1.5 Mio. cells/ml; 1.5ml
Human Colon adenocarcinoma cell line SW-480

Organism: Homo sapiens (human)
Ethnicity: Caucasian
Age: 51 years old
Gender: Male
Tissue: Colon, Grade IV, Dukes' type B
Morphology: Epithelial
Celltype: Adenocarcinoma, colorectal
Growth Properties: Monolayer, adherent
Description: The SW480 cell line originated from a surgical specimen of a primary tumor of a moderately differentiated colon adenocarcinoma. The line is negative for CSAp (CSAp-) and colon antigen 3. The cells are positive for keratin by immunoperoxidase staining. There is a G -> A mutation in codon 273 of the p53 gene resulting in an Arg -> His substitution and a C -> T mutation in codon 309 resulting in a Pro -> Ser substitution. The cells express elevated levels of p53 protein. The line is positive for expression of c-myc, K-ras, H-ras, N-ras, myb, sis and fos oncogenes. N-myc oncogene expression was not detected. Matrilysin, a metalloproteinase associated with tumor invasiveness, is not expressed. The cells have been reported to produce GM-CSF. This line has a mutation in codon 12 of the ras protooncogene, and can be used as a positive control for PCR assays of mutation in this codon.