Artikelnummer: 300457

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Human Colon Carcinoma cell line
Frozen cryovial
3 Mio. cells/ml; 1.5ml
Human Colon Carcinoma cell line LS-513

Organism: Homo sapiens (human)
Ethnicity: Caucasian
Age: 63 years
Gender: Male
Tissue: Colorectal carcinoma, cecum, Dukes' type C
Morphology: Epithelial
Growth Properties: Monolayer, adherent
Description: LS-513 is a colorectal carcinoma cell line isolated in 1985 from a primary tumor biopsy from a 63 year old Caucasian male patient diagnosed with a Dukes' C mucin secreting cecal tumor located at the Bauhin valve. Approximately 50% of LS-513 cells express surface carcinoembryonic antigen (CEA). LS-513 cells express the major histocompatibility (MHC) class I antigens HLA and beta 2 microglobulin. MHC class II antigens (HLA-DR, DQ, and DP were not detected). TGF beta-1 is inhibitory for proliferation of LS-513 cells, whereas TGF beta-2 has no effect on the growth of these cells. LS-513 cells are 100-fold less sensitive to TGF beta-1 than the LS-1034 (ATCC CRL-2158) cell line. LS-513 cells are multidrug resistant (MDR) and are tumorigenic in nude mice. Colony forming efficiency was 30% in methylcellulose.