A-549 heat shock Cell Lysate

Artikelnummer: 300114CLHS

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Zelllysat aus A-549 Zellens
A-549 - Humane Lungenkarzinom Zellen
Hitzeschock-behandelt (heat shock cell lysate)
A-549 heat shock cell lysate
Whole Cell Lysate, isolated from A-549 human Lung Carcinoma cells

Heat shock pretreatment: 42?C for 1 hr, 37?C for 3 hrs

The lysate comes in RIPA buffer.

Western Blotting (SDS PAGE)

For Western blotting, the lysates must be boiled for 5 min at 95?C in SDS gel loading buffer.

Lysates should be kept on ice or at 4?C at all times.
Do not allow to stand at ambient or higher temperatures.
Long-term storage at -80?C is recommended.
Several freeze-thaw cycles must be avoided.