A-549 heat shock Cell Lysate

Artikelnummer: 300114CLHS

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Produktinformationen

Zelllysat aus A-549 Zellens
A-549 - Humane Lungenkarzinom Zellen
Hitzeschock-behandelt (heat shock cell lysate)
A-549 heat shock cell lysate
Whole Cell Lysate, isolated from A-549 human Lung Carcinoma cells

Heat shock pretreatment: 42?C for 1 hr, 37?C for 3 hrs

The lysate comes in RIPA buffer.

Application:
Western Blotting (SDS PAGE)
Immunoprecipitation

For Western blotting, the lysates must be boiled for 5 min at 95?C in SDS gel loading buffer.

Storage:
Lysates should be kept on ice or at 4?C at all times.
Do not allow to stand at ambient or higher temperatures.
Long-term storage at -80?C is recommended.
Several freeze-thaw cycles must be avoided.