HEK293 Heat shock cell lysate

Artikelnummer: 300192CLHS

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Produktinformationen

Whole Cell Lysate from heat shock treated HEK293 cells
Human Embryo kidney cells
100 ?g in RIPA buffer
HEK293 heat shock cell lysate
Human Embryo kidney cell lysate

Heat shock pretreatment: 42?C for 1 hr, 37?C for 3 hrs.

The lysate comes in RIPA buffer.

Application:
Western Blotting (SDS PAGE)
Immunoprecipitation

For Western blotting, the lysates must be boiled for 5 min at 95?C in a SDS containing gel loading buffer.

Storage:
Lysates should be kept on ice or at 4?C at all times.
Do not allow to stand at ambient or higher temperatures.
Long-term storage at -80?C is recommended.
Several freeze-thaw cycles must be avoided.