RPMI 1640 medium kit hPL
69,95 € je 250ml
zzgl. MwSt. zzgl Versandkosten
250 ml medium, basic
12.5 ml hPL supplement (5% final), deep-frozen
RPMI 1640 cell culture medium supplemented with hPL (human Platelate lysate)
RPMI-1640 represents an excellent cell culture medium for human and animal lymphocytes, either normal or neoplastic. The RPMI media were developed at the Roswell Park Memorial Institute, Buffalo, N.Y., USA and meanwhile a variety of culture media based on RPMI are in use.
The supplement hPL (human Platelet lysate) is manufactured from platelet single donor apheresis samples which are collected at blood banks in Southern Germany. Contrary to FBS, hPL is a bystander product. Platelet preparations must be destroyed 5 days after collection from the donors unless they are used up in other applications. Only platelets from double-negative tested donors are used to manufacture the platelet lysates and are pooled from 25 donors.
RPMI 1640 medium supplemented with hPL delivers sufficient nutrients and growth factors to sustain the proliferation of cell lines such as Jurkat cells.