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supplemented with 4.5g/L glucose, L-glutamine
Addition of serum or other supplementation required
RPMI-1640 represents an excellent cell culture medium for human and animal lymphocytes, either normal or neoplastic. Murine myeloma cells proliferate in this medium as well, if it is supplemented with 15% serum. Selection media based on RPMI 1640 for the generation of hybridoma are supplemented with HAT or HT.
The RPMI media were developed at the Roswell Park Memorial Institute, Buffalo, N.Y., USA and meanwhile a variety of culture media based on RPMI are in use.
This basic RPMI 1640 High glucose must be supplemented with serum or hPL to promote cell proliferation.