Basic Medium



Hier finden Sie alle Medien, die für die Kultivierung der bei CLS GmbH erhältlichen Zellen und Zelllinien verwendet werden.
Die meisten der Medien sind mit L-Glutamin supplementiert.
Bitte beachten Sie, dass die Basic Medien vor Gebrauch supplementiert werden müssen, entweder mit FBS oder mit hPL (Plättchenlysat) oder einem serumfreien Supplement.

Artikelübersicht

13 Artikel in dieser Kategorie gefunden

  • RPMI 1640, ohne Phenolrot

    Nummer: 820706a

    Bestand: Sofort lieferbar
    RPMI 1640 Zellkulturmedium
    Basic
    Enthält kein Phenolrot

    Das Basismedium muss supplementiert werden den Bedingungen für die betreffende Zelllinie.
  • DMEM-HG, basic

    Nummer: 820300a

    Bestand: Sofort lieferbar
    DMEM (Dulbecco’s Modified Eagle’s Medium) contains four-fold concentrations of the amino acids and vitamins as present in BME (Basal Medium Eagle). This culture medium, enriched with 4.5 g/L glucose, supports the proliferation of a wide variety of normal and transformed cells, such as primary cultures of mouse and chicken cells growing adherently.
  • DMEM:Ham's F12 (1:1), for NCI-H295R, basic

    Nummer: 820402a

    Bestand: 3-5 Tage Lieferzeit
    The DMEM:Ham’s F12 is a 1:1 mixture of DMEM containing 4.5 g/L glucose and Ham’s F12. As this medium is rich in amino acids, hormones and minerals, it supports the growth of many cells and cell lines even under low serum conditions. Thus, it is possible to work with serum concentrations of 5% or less.
    This special medium has been adapted to support the proliferation of NCI-H295R cells.
    The final concentration of bovine serum is below 1%.
  • DMEM:Ham's F12, basic

    Nummer: 820400a

    Bestand: Sofort lieferbar
    The DMEM:Ham’s F12 is a 1:1 mixture of DMEM containing 4.5 g/L glucose and Ham’s F12. As this medium is rich in amino acids, hormones and minerals, it supports the growth of many cells and cell lines even under low serum conditions. Thus, it is possible to work with serum concentrations of 5% or less. Furthermore, it is an excellent basic medium for the serum free cell culture.
  • EMEM completed, basic

    Nummer: 820100c

    Bestand: 3-5 Tage Lieferzeit
    EMEM (Minimum Essential Medium Eagle) represents a cell culture medium which was developed from BME (Basal Medium Eagle), suited for the propagation of a wide variety of cell lines.
    Cell growth is supported due to the addition of L-Glutamine, NEAA and pyruvate.
    This culture medium is recommended for verioius adherent cell types.
  • EMEM, basic

    Nummer: 820100a

    Bestand: Sofort lieferbar
    EMEM (Minimum Essential Medium Eagle) represents a cell culture medium which was developed from BME (Basal Medium Eagle), suited for the propagation of a wide variety of cell lines. Cell growth is supported due to the addition of L-Glutamine. This culture medium is recommended for adherent cell types.
  • Ham's F12 medium, basic

    Nummer: 820600a

    Bestand: Sofort lieferbar
    Ham's F12 cell culture medium was formulated to support the serum free culture of CHO cells. It is also suitable for the culture of carcinoma cells, rat skeletal myoblasts, Chinese hamster lung cells, as well as rat, rabbit, and chicken embryos.
  • McCoy's 5a, basic

    Nummer: 820200a

    Bestand: Sofort lieferbar
    This medium was developed originally to culture Novikoff Hepatoma cells and successfully applied in the proliferation of a wide range of human and rat normal and transformed cells. The composition modified by Iwakata and Grace (Iwakata. S and JT Grace. N.Y. State J. Med. 64: 2279, 1964) includes increased levels of inositol and glucose and contains glutamine.
  • Medium 199, basic

    Nummer: 820101a

    Bestand: 3-5 Tage Lieferzeit
    Medium 199 described in 1950 for the first time and intended for the culture of chick embryo fibroblasts represents a very complex cell culture medium. It contains unique components such as adenine, adenosine, thymine and hypoxanthine. Recommended for the culture of non-transformed cells. The supplementation with Earles salts requires the incubation in 5-10% CO2 atmosphere.
  • RPMI 1640, basic

    Nummer: 820700a

    Bestand: Sofort lieferbar
    RPMI-1640 represents an excellent cell culture medium for human and animal lymphocytes, either normal or neoplastic. Murine myeloma cells proliferate in this medium as well, if it is supplemented with 15% serum. Selection media based on RPMI 1640 for the generation of hybridoma are supplemented with HAT or HT.
    The RPMI media were developed at the Roswell Park Memorial Institute, Buffalo, N.Y., USA and meanwhile a variety of culture media based on RPMI are in use.