RPMI 1640 Zellkulturmedium
Basic
Enthält kein Phenolrot

Das Basismedium muss supplementiert werden den Bedingungen für die betreffende Zelllinie.

DMEM (Dulbecco’s Modified Eagle’s Medium) contains four-fold concentrations of the amino acids and vitamins as present in BME (Basal Medium Eagle). This culture medium, enriched with 4.5 g/L glucose, supports the proliferation of a wide variety of normal and transformed cells, such as primary cultures of mouse and chicken cells growing adherently.

The DMEM:Ham’s F12 is a 1:1 mixture of DMEM containing 4.5 g/L glucose and Ham’s F12. As this medium is rich in amino acids, hormones and minerals, it supports the growth of many cells and cell lines even under low serum conditions. Thus, it is possible to work with serum concentrations of 5% or less.
This special medium has been adapted to support the proliferation of NCI-H295R cells.
The final concentration of bovine serum is below 1%.

The DMEM:Ham’s F12 is a 1:1 mixture of DMEM containing 4.5 g/L glucose and Ham’s F12. As this medium is rich in amino acids, hormones and minerals, it supports the growth of many cells and cell lines even under low serum conditions. Thus, it is possible to work with serum concentrations of 5% or less. Furthermore, it is an excellent basic medium for the serum free cell culture.

EMEM (Minimum Essential Medium Eagle) represents a cell culture medium which was developed from BME (Basal Medium Eagle), suited for the propagation of a wide variety of cell lines.
Cell growth is supported due to the addition of L-Glutamine, NEAA and pyruvate.
This culture medium is recommended for verioius adherent cell types.

EMEM (Minimum Essential Medium Eagle) represents a cell culture medium which was developed from BME (Basal Medium Eagle), suited for the propagation of a wide variety of cell lines. Cell growth is supported due to the addition of L-Glutamine. This culture medium is recommended for adherent cell types.

Ham's F12 cell culture medium was formulated to support the serum free culture of CHO cells. It is also suitable for the culture of carcinoma cells, rat skeletal myoblasts, Chinese hamster lung cells, as well as rat, rabbit, and chicken embryos.

Ham's F12 cell culture medium was formulated to support the serum free culture of CHO cells. It is also suitable for the culture of carcinoma cells, rat skeletal myoblasts, Chinese hamster lung cells, as well as rat, rabbit, and chicken embryos.

IMEM
supplemented with L-glutamine
without FBS

This medium was developed originally to culture Novikoff Hepatoma cells and successfully applied in the proliferation of a wide range of human and rat normal and transformed cells. The composition modified by Iwakata and Grace (Iwakata. S and JT Grace. N.Y. State J. Med. 64: 2279, 1964) includes increased levels of inositol and glucose and contains glutamine.

Medium 199 described in 1950 for the first time and intended for the culture of chick embryo fibroblasts represents a very complex cell culture medium. It contains unique components such as adenine, adenosine, thymine and hypoxanthine. Recommended for the culture of non-transformed cells. The supplementation with Earles salts requires the incubation in 5-10% CO2 atmosphere.

RPMI-1640 represents an excellent cell culture medium for human and animal lymphocytes, either normal or neoplastic. Murine myeloma cells proliferate in this medium as well, if it is supplemented with 15% serum. Selection media based on RPMI 1640 for the generation of hybridoma are supplemented with HAT or HT.
The RPMI media were developed at the Roswell Park Memorial Institute, Buffalo, N.Y., USA and meanwhile a variety of culture media based on RPMI are in use.