HEK293 Heat shock cell lysate

Artikelnummer: 300192CLHS

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Produktinformationen

Whole Cell Lysate from heat shock treated HEK293 cells
Human Embryo kidney cells
100 g in RIPA buffer
HEK293 heat shock cell lysate
Human Embryo kidney cell lysate

Heat shock pretreatment: 42C for 1 hr, 37C for 3 hrs.

The lysate comes in RIPA buffer.

Application:
Western Blotting (SDS PAGE)
Immunoprecipitation

For Western blotting, the lysates must be boiled for 5 min at 95C in a SDS containing gel loading buffer.

Storage:
Lysates should be kept on ice or at 4C at all times.
Do not allow to stand at ambient or higher temperatures.
Long-term storage at -80C is recommended.
Several freeze-thaw cycles must be avoided.